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Cam EFLS A. J. Kabla

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Quantification of Morphogenetic Reorganisations

 

Work done in collaboration with Richard Adams' lab (Department of Physiology, Development and Neurosciences, Cambridge) and L. Mahadevan (Harvard University).


The shape evolution of living tissue is primarily determined by cell motility and cell adhesion. Although cell reorganization is unambiguously defined at the cell scale by discrete neighbor changes, key morphogenetic processes become measurable only at an intermediate spatial scale. Tools that span cellular and mesoscopic scales are thus required in order to relate tissue-wide deformations to the fundamental underlying cellular mechanisms that drive morphogenesis. We introduce a comprehensive framework for quantifying the mesoscale kinematics of morphogenesis. Using simultaneously information from cell displacements and cell shape changes, we quantitatively distinguish the contributions of individual cell deformation and neighbor change to the global deformation field. This framework is tested on real and simulated data, and allowed the direct evaluation of rheological models for soft living tissues.

 

References:
Tissue tectonics: morphogenetic strain rates, cell shape change and intercalation
G.B. Blanchard, A.J. Kabla, N.L. Schultz, L.C. Butler, B. Sanson, N. Gorfinkiel, L. Mahadevan and R.J. Adams
Nature Methods, 6, 458-464 (2009), DOI:10.1038/NMETH.1327.
Cell shape changes indicate a role for extrinsic tensile forces in Drosophila germ-band extension
L.C. Butler, G.B. Blanchard, A.J. Kabla, N.J. Lawrence, D.P. Welchman, L. Mahadevan, R.J. Adams and B. Sanson
Nature Cell Biology, 2009 11(7):859-64.
Bridging cell and tissue behaviour in embryomorphogenesis
A.J. Kabla, G.B. Blanchard, R.J. Adams and L. Mahadevan
Book chapter in Cell Mechanics: From Single Scale-Based Models to Multiscale Modeling, Taylor and Francis ed., 2010.